Hotspot

ndhF-rpl32

SSC · 110339-110832 · 494 bp

Back to Kalanchoe

Pi 0.0357

MarkerSeek score 70.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 494
variable_sites: 58
pi: 0.0357
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGAACCGGAAGTGGAAGTA	TTCGAACCGAGATGCTCAAG	50.0	50.0	58.0	58.0	1333-1389 bp	1.0	91.1
ndhF-rpl32_p2	AGGAACCGGAAGTGGAAGTA	TTAGAAGAGAATGTGCCGCC	50.0	50.0	58.0	58.0	1360-1416 bp	1.0	90.9
ndhF-rpl32_p3	AGGAACCGGAAGTGGAAGTA	AAGCACTGCTTCCTAAGAGC	50.0	50.0	58.0	57.9	1316-1372 bp	1.0	90.4
ndhF-rpl32_p4	CCAAAATCCCCGATACGGTT	TTCGAACCGAGATGCTCAAG	50.0	50.0	58.0	58.0	1839-1895 bp	1.0	89.7
ndhF-rpl32_p5	CCAAAATCCCCGATACGGTT	TTAGAAGAGAATGTGCCGCC	50.0	50.0	58.0	58.0	1866-1922 bp	1.0	89.5