Hotspot

petA-psbJ

LSC · 61811-62661 · 851 bp

Back to Kalanchoe

Pi 0.036

MarkerSeek score 71.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 94
pi: 0.036
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGAAACAGTTTGAGAAGGTTCA	CAGGATTGGGTTCATCCTTGT	36.4	47.6	55.8	57.9	710-931 bp	1.0	52.0
petA-psbJ_p2	AGAAACAGTTTGAGAAGGTTCA	TCAGGATTGGGTTCATCCTTG	36.4	47.6	55.8	57.6	711-932 bp	1.0	52.0
petA-psbJ_p3	AAGAAACAGTTTGAGAAGGTTCA	CAGGATTGGGTTCATCCTTGT	34.8	47.6	56.5	57.9	711-932 bp	1.0	52.0
petA-psbJ_p4	AGAAACAGTTTGAGAAGGTTCAA	CAGGATTGGGTTCATCCTTGT	34.8	47.6	56.5	57.9	710-931 bp	1.0	52.0
petA-psbJ_p5	TGAGAAGGTTCAATTATCCGA	CAGGATTGGGTTCATCCTTGT	38.1	47.6	54.4	57.9	700-921 bp	1.0	52.0