Hotspot

rbcL-accD

LSC · 55781-56438 · 658 bp

Back to Kalanchoe

Pi 0.0274

MarkerSeek score 69.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 64
pi: 0.0274
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTTGAATTTGAAGCAATGGAT	CAATAGGCCCCAGATTGTCC	28.6	55.0	52.5	58.0	596-807 bp	1.0	51.2
rbcL-accD_p2	TGAATTTGAAGCAATGGATAC	CAATAGGCCCCAGATTGTCC	33.3	55.0	52.5	58.0	594-805 bp	1.0	51.2
rbcL-accD_p3	ATTTGAATTTGAAGCAATGGA	GCCCCAGATTGTCCATTGAT	28.6	50.0	52.5	57.9	591-802 bp	1.0	51.2
rbcL-accD_p4	TTTGAATTTGAAGCAATGGAT	GCCCCAGATTGTCCATTGAT	28.6	50.0	52.5	57.9	590-801 bp	1.0	51.2
rbcL-accD_p5	ATTTGAATTTGAAGCAATGGA	CAATAGGCCCCAGATTGTCC	28.6	55.0	52.5	58.0	597-808 bp	1.0	51.1