Hotspot

ndhC-trnV-UAC

LSC · 49826-50685 · 860 bp

Back to Kalanchoe

Pi 0.0206

MarkerSeek score 69.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 55
pi: 0.0206
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCTACGATCGGAATAACGCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.0	57.8	700-1000 bp	0.928571	83.8
ndhC-trnV-UAC_p2	ACGATCGGAATAACGCTTGA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.4	57.8	697-997 bp	1.0	82.7
ndhC-trnV-UAC_p3	TGCTACGATCGGAATAACGC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	701-1001 bp	0.928571	82.3
ndhC-trnV-UAC_p4	GCTACGATCGGAATAACGCT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.0	57.5	699-999 bp	0.928571	81.1
ndhC-trnV-UAC_p5	GCTACGATCGGAATAACGCT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.0	58.7	688-988 bp	0.928571	80.0