Hotspot

trnS-GGA-rps4

LSC · 44962-45174 · 213 bp

Back to Kalanchoe

Pi 0.0458

MarkerSeek score 66.50

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 213
variable_sites: 25
pi: 0.0458
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	TTCGAATCCCTCTCTTTCCG	AGTAAAGGGGTCGGTTTGAA	50.0	45.0	57.0	56.7	269-299 bp	0.928571	70.3
trnS-GGA-rps4_p2	GGGTTCGAATCCCTCTCTTT	AGTAAAGGGGTCGGTTTGAA	50.0	45.0	56.9	56.7	272-302 bp	0.928571	69.3
trnS-GGA-rps4_p3	GGGTTCGAATCCCTCTCTTTC	AGTAAAGGGGTCGGTTTGAA	52.4	45.0	58.1	56.7	272-302 bp	0.928571	69.2
trnS-GGA-rps4_p4	GGTTCGAATCCCTCTCTTTCC	AGTAAAGGGGTCGGTTTGAA	52.4	45.0	58.1	56.7	271-301 bp	0.928571	69.2
trnS-GGA-rps4_p5	TTCGAATCCCTCTCTTTCCG	AGTAAAGGGGTCGGTTTGAAA	50.0	42.9	57.0	57.4	269-299 bp	0.928571	68.3