Hotspot

lhbA-trnG-UCC

LSC · 35974-36617 · 644 bp

Back to Kalanchoe

Pi 0.0419

MarkerSeek score 72.60

Primer available yes

Label lhbA-trnG-UCC

Feature Metadata

label_name: lhbA-trnG-UCC
feature_type: igs
region: LSC
length_bp: 644
variable_sites: 71
pi: 0.0419
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
lhbA-trnG-UCC_p1	CAACCACTCAGCCATCTCTC	CGTTGTTTTTCGACCGGAAG	55.0	50.0	58.0	58.0	1303-1784 bp	1.0	89.9
lhbA-trnG-UCC_p2	CAACCACTCAGCCATCTCTC	CAATCCCCACCACGGAATAG	55.0	55.0	58.0	58.0	1345-1826 bp	1.0	89.6
lhbA-trnG-UCC_p3	GAGCTATCAACCACTCAGCC	CGTTGTTTTTCGACCGGAAG	55.0	50.0	58.1	58.0	1310-1791 bp	1.0	89.6
lhbA-trnG-UCC_p4	ACCGGGCCTGTAAAAACAAT	CGTTGTTTTTCGACCGGAAG	45.0	50.0	58.0	58.0	1424-1897 bp	0.714286	78.2
lhbA-trnG-UCC_p5	ACCGGGCCTGTAAAAACAAT	CAATCCCCACCACGGAATAG	45.0	55.0	58.0	58.0	1466-1939 bp	0.714286	78.0