Hotspot

trnE-UUC-trnT-GGU

LSC · 31296-32047 · 752 bp

Back to Kalanchoe

Pi 0.0311

MarkerSeek score 68.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 71
pi: 0.0311
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	666-885 bp	1.0	89.1
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	645-864 bp	1.0	89.1
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	624-843 bp	1.0	88.9
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	645-864 bp	1.0	88.8
trnE-UUC-trnT-GGU_p5	TTCCTAGTACCCTACCCCCA	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	703-922 bp	1.0	88.6