Hotspot

trnK-UUU-rps16

LSC · 4187-4863 · 677 bp

Back to Kalanchoe

Pi 0.033

MarkerSeek score 72.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 71
pi: 0.033
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCGAATTGGAGCTAAAGGGC	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.0	58.0	1617-1673 bp	1.0	88.0
trnK-UUU-rps16_p2	CGAATTGGAGCTAAAGGGCT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.0	58.0	1616-1672 bp	1.0	88.0
trnK-UUU-rps16_p3	TGGCTTTTTGTTTCCCTCGA	AAAAGGGTATGTTGCTGCCA	45.0	45.0	57.9	58.0	1633-1689 bp	1.0	87.8
trnK-UUU-rps16_p4	TCAGTCGTGGTCGTACAAAC	AAAAGGGTATGTTGCTGCCA	50.0	45.0	57.9	58.0	1449-1500 bp	1.0	87.8
trnK-UUU-rps16_p5	GTTTCCCTCGAATTGGAGCT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	57.9	58.0	1624-1680 bp	1.0	87.4