Hotspot

rpl14-rpl16

LSC · 83757-83915 · 159 bp

Back to Kageneckia

Pi 0.0126

MarkerSeek score 66.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 3
pi: 0.0126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCGCTGTTATCCGCTACATT	TGAAATGAGCGGAGTAGCAG	50.0	50.0	58.1	57.7	297-300 bp	1.0	80.0
rpl14-rpl16_p2	TACACATCAATTCTCGGGCC	TGAAATGAGCGGAGTAGCAG	50.0	50.0	57.7	57.7	317-320 bp	1.0	78.3
rpl14-rpl16_p3	CCGCTGTTATCCGCTACATT	GAAATGAGCGGAGTAGCAGA	50.0	50.0	58.1	57.4	296-299 bp	1.0	77.7
rpl14-rpl16_p4	TACACATCAATTCTCGGGCC	GAAATGAGCGGAGTAGCAGA	50.0	50.0	57.7	57.4	316-319 bp	1.0	76.0
rpl14-rpl16_p5	TCCGCTACATTCAAATGGGT	TGAAATGAGCGGAGTAGCAG	45.0	50.0	57.2	57.7	288-291 bp	1.0	74.8