Hotspot

rps8-rpl14

LSC · 83209-83387 · 179 bp

Back to Kageneckia

Pi 0.0186
MarkerSeek score 64.40
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
179
variable_sites
5
pi
0.0186
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 TGTCAGCATTTCGTATAGAGGT GCCCGGGAATTAAGACAGTT 40.9 50.0 57.0 57.9 294-304 bp 1.0 56.3
rps8-rpl14_p2 TGTCAGCATTTCGTATAGAGGTT GCCCGGGAATTAAGACAGTT 39.1 50.0 57.7 57.9 294-304 bp 1.0 53.6
rps8-rpl14_p3 TGTCAGCATTTCGTATAGAGGT GATCGCCCGGGAATTAAGAC 40.9 55.0 57.0 58.5 298-308 bp 1.0 53.4
rps8-rpl14_p4 ATGTCAGCATTTCGTATAGAGGT GCCCGGGAATTAAGACAGTT 39.1 50.0 57.5 57.9 295-305 bp 1.0 52.0
rps8-rpl14_p5 TGTCAGCATTTCGTATAGAGGTT GATCGCCCGGGAATTAAGAC 39.1 55.0 57.7 58.5 298-308 bp 1.0 50.7