Hotspot

psbF-psbE

LSC · 66808-66816 · 9 bp

Back to Kageneckia

Pi 0.0741
MarkerSeek score 75.00
Primer available yes
Label psbF-psbE

Feature Metadata

label_name
psbF-psbE
feature_type
igs
region
LSC
length_bp
9
variable_sites
1
pi
0.0741
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbE_p1 GGTAGGTACAGCTAGTCCGT AACTGGCCGTTTTGATCCTT 55.0 45.0 58.0 58.0 136 bp 1.0 80.2
psbF-psbE_p2 GGTAGGTACAGCTAGTCCGT ACTGGCCGTTTTGATCCTTT 55.0 45.0 58.0 58.0 135 bp 1.0 80.2
psbF-psbE_p3 ACGGTAGGTACAGCTAGTCC AACTGGCCGTTTTGATCCTT 55.0 45.0 58.0 58.0 138 bp 1.0 80.1
psbF-psbE_p4 ACGGTAGGTACAGCTAGTCC ACTGGCCGTTTTGATCCTTT 55.0 45.0 58.0 58.0 137 bp 1.0 80.1
psbF-psbE_p5 GTCCGTGAACAGCTAACCAT ACTGGCCGTTTTGATCCTTT 50.0 45.0 57.9 58.0 121 bp 1.0 79.9