Hotspot

psaI-ycf4

LSC · 61908-62318 · 411 bp

Back to Kageneckia

Pi 0.0138

MarkerSeek score 52.00

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 411
variable_sites: 8
pi: 0.0138
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TTTCGCTTTCTTCCGTGGAA	GTTTTTCGAGACCCCGCTAT	45.0	50.0	58.0	58.0	1028-1136 bp	1.0	85.3
psaI-ycf4_p2	TTTCGCTTTCTTCCGTGGAA	TCGAGACCCCGCTATAAGTT	45.0	50.0	58.0	57.9	1023-1131 bp	1.0	84.9
psaI-ycf4_p3	TTTCGCTTTCTTCCGTGGAA	TTCGAGACCCCGCTATAAGT	45.0	50.0	58.0	57.9	1024-1132 bp	1.0	84.9
psaI-ycf4_p4	TTTCGCTTTCTTCCGTGGAA	CGGAGGAATATGCGACGATT	45.0	50.0	58.0	57.9	1328-1436 bp	1.0	84.3
psaI-ycf4_p5	TTTCGCTTTCTTCCGTGGAA	GCTCGAGTAAGCGTTCTGAA	45.0	50.0	58.0	58.0	1636-1743 bp	1.0	83.7