Hotspot

ndhC-trnV-UAC

LSC · 53031-53660 · 630 bp

Back to Kageneckia

Pi 0.0313

MarkerSeek score 75.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 630
variable_sites: 27
pi: 0.0313
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	825-859 bp	1.0	84.1
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	761-795 bp	1.0	84.1
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	ATGCACGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	827-861 bp	1.0	83.0
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	AATGCACGTTGGGTCTTTGA	50.0	45.0	57.9	58.3	828-862 bp	1.0	82.9
ndhC-trnV-UAC_p5	CCTTTGCTAATCGGGGCTAA	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.7	58.2	837-871 bp	1.0	82.5