Hotspot

trnF-GAA-ndhJ

LSC · 50795-51337 · 543 bp

Back to Kageneckia

Pi 0.00737

MarkerSeek score 62.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 543
variable_sites: 6
pi: 0.00737
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	717-874 bp	1.0	80.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	681-838 bp	1.0	79.7
trnF-GAA-ndhJ_p3	GTCGTGAATGGTCGGGATAG	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	730-887 bp	1.0	79.0
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	705-862 bp	1.0	79.0
trnF-GAA-ndhJ_p5	GAGGATGATGCGTCGTGAAT	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	741-898 bp	1.0	78.9