Hotspot

psbZ-trnG-GCC

LSC · 38381-38868 · 488 bp

Back to Kageneckia

Pi 0.0148

MarkerSeek score 61.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 9
pi: 0.0148
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	600-646 bp	1.0	81.6
psbZ-trnG-GCC_p2	GGGTTGGTCGGGTAACAAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	586-632 bp	1.0	81.3
psbZ-trnG-GCC_p3	TTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.0	57.8	556-602 bp	1.0	75.7
psbZ-trnG-GCC_p4	AGGGTTGGTCGGGTAACAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	59.2	57.8	587-633 bp	1.0	74.6
psbZ-trnG-GCC_p5	ATTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.2	57.8	601-647 bp	1.0	74.2