Hotspot

trnR-UCU-atpA

LSC · 10235-10756 · 522 bp

Back to Kageneckia

Pi 0.0226

MarkerSeek score 63.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 522
variable_sites: 12
pi: 0.0226
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	517-733 bp	1.0	69.9
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	516-732 bp	1.0	69.9
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	47.6	50.0	57.3	58.4	456-672 bp	1.0	69.1
trnR-UCU-atpA_p4	TGGATAGGACAGAGGTCTTCT	AGACATTTACCGACGAAGCG	47.6	50.0	56.9	58.4	491-707 bp	1.0	66.1
trnR-UCU-atpA_p5	TGGAATGAAAAGCGTCCATT	AGACATTTACCGACGAAGCG	40.0	50.0	55.9	58.4	517-733 bp	1.0	65.9