Hotspot

trnK-UUU-rps16

LSC · 4320-5304 · 985 bp

Back to Kageneckia

Pi 0.014

MarkerSeek score 56.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 985
variable_sites: 18
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	CGGAACTTCGCCCTAATCAA	55.0	50.0	58.0	58.0	1147-1157 bp	1.0	83.7
trnK-UUU-rps16_p2	TCAGGATCAGTCGTGGTCTT	CGGAACTTCGCCCTAATCAA	50.0	50.0	58.1	58.0	1143-1153 bp	1.0	83.2
trnK-UUU-rps16_p3	TTCAGGATCAGTCGTGGTCT	CGGAACTTCGCCCTAATCAA	50.0	50.0	58.1	58.0	1144-1154 bp	1.0	83.2
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	CGACGAGAGGGAAGAGATCT	55.0	55.0	58.0	58.0	1301-1311 bp	1.0	83.1
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	GCCGTACGAGGAGAAAACTT	55.0	50.0	58.0	57.9	1403-1414 bp	1.0	82.7