Hotspot

ndhF

SSC · 117833-120061 · 2229 bp

Back to Kaempferia

Pi 0.00547

MarkerSeek score 47.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 23
pi: 0.00547
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGCGGTACCCATCCTTTTAT	ACAAGTACAAGAAAGGAAGGA	50.0	38.1	57.8	54.4	2920-2969 bp	1.0	44.2
ndhF_p2	CAATATCGCGGTACCCATCC	ACAAGTACAAGAAAGGAAGGA	55.0	38.1	58.3	54.4	2926-2975 bp	1.0	43.9
ndhF_p3	AATATCGCGGTACCCATCCT	ACAAGTACAAGAAAGGAAGGA	50.0	38.1	58.4	54.4	2925-2974 bp	1.0	43.3
ndhF_p4	ATATCGCGGTACCCATCCTT	ACAAGTACAAGAAAGGAAGGA	50.0	38.1	58.4	54.4	2924-2973 bp	1.0	43.3
ndhF_p5	CGCGGTACCCATCCTTTTAT	ACAAGTACAAGAAAGGAAGGAA	50.0	36.4	57.8	55.2	2920-2969 bp	1.0	43.0