Hotspot

ndhF-rpl32

SSC · 120062-120840 · 779 bp

Back to Kaempferia

Pi 0.0283

MarkerSeek score 64.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 779
variable_sites: 33
pi: 0.0283
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCGGTCGTGTGAACCAAAAT	TTGAACGTCCGGTAGAAACC	45.0	50.0	58.0	57.8	1302-1417 bp	1.0	84.9
ndhF-rpl32_p2	TACATGAAGGGGGAATTGCG	TTGAACGTCCGGTAGAAACC	50.0	50.0	57.9	57.8	1538-1653 bp	1.0	84.5
ndhF-rpl32_p3	TCGGTCGTGTGAACCAAAAT	TTCGGGGTATGTCAATTCCG	45.0	50.0	58.0	57.7	1367-1482 bp	1.0	83.5
ndhF-rpl32_p4	TACATGAAGGGGGAATTGCG	TTCGGGGTATGTCAATTCCG	50.0	50.0	57.9	57.7	1603-1718 bp	1.0	83.2
ndhF-rpl32_p5	TTAGCAACTGCACCGAGAAA	TTGAACGTCCGGTAGAAACC	45.0	50.0	57.8	57.8	1513-1628 bp	1.0	82.9