Hotspot

ycf1-ndhF

SSC · 117814-117832 · 19 bp

Back to Kaempferia

Pi 0.368

MarkerSeek score 72.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 19
variable_sites: 13
pi: 0.368
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	CGCGGTACCCATCCTTTTAT	TCCCAATGCGAGTTATTCCG	50.0	50.0	57.8	58.1	851-893 bp	1.0	84.5
ycf1-ndhF_p2	CAATATCGCGGTACCCATCC	TCCCAATGCGAGTTATTCCG	55.0	50.0	58.3	58.1	857-899 bp	1.0	84.3
ycf1-ndhF_p3	AATATCGCGGTACCCATCCT	TCCCAATGCGAGTTATTCCG	50.0	50.0	58.4	58.1	856-898 bp	1.0	83.6
ycf1-ndhF_p4	ATATCGCGGTACCCATCCTT	TCCCAATGCGAGTTATTCCG	50.0	50.0	58.4	58.1	855-897 bp	1.0	83.6
ycf1-ndhF_p5	TCGAAGGTCAATGCACAACT	TCCCAATGCGAGTTATTCCG	45.0	50.0	57.7	58.1	1118-1160 bp	1.0	83.1