Hotspot

rpl22-rps19

IRb · 87907-88156 · 250 bp

Back to Kaempferia

Pi 0.029
MarkerSeek score 63.60
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
IRb
length_bp
250
variable_sites
13
pi
0.029
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 GCCCTTTGAAAACAGACAT TACGAGACACGCAAGAAACG 42.1 50.0 53.7 58.6 292-331 bp 1.0 47.3
rpl22-rps19_p2 GCCCTTTGAAAACAGACAT GTGGGTCACAAATTGGGAGA 42.1 50.0 53.7 57.7 333-372 bp 1.0 47.0
rpl22-rps19_p3 GCCCTTTGAAAACAGACAT ATGGTGGGTCACAAATTGGG 42.1 50.0 53.7 58.4 336-375 bp 1.0 46.9
rpl22-rps19_p4 GCCCTTTGAAAACAGACAT GATCGTATGGTGGGTCACAA 42.1 50.0 53.7 57.3 342-381 bp 1.0 46.9
rpl22-rps19_p5 GCCCTTTGAAAACAGACAT TAACGGATCGTATGGTGGGT 42.1 50.0 53.7 58.2 347-386 bp 1.0 46.8