Hotspot

psbC-trnS-UGA

LSC · 37902-38050 · 149 bp

Back to Kaempferia

Pi 0.0302

MarkerSeek score 73.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 149
variable_sites: 9
pi: 0.0302
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CTTTCCATGACCCCTCTTCG	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.0	58.0	261-265 bp	1.0	83.9
psbC-trnS-UGA_p2	CATTTGTGGCATGCAGGAAG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	339-343 bp	1.0	83.1
psbC-trnS-UGA_p3	CATTTGTGGCATGCAGGAAG	GCTGAGTGGTTGATAGCTCC	50.0	55.0	58.0	58.1	331-335 bp	1.0	82.9
psbC-trnS-UGA_p4	CATTTGTGGCATGCAGGAAG	GGCTGAGTGGTTGATAGCTC	50.0	55.0	58.0	58.1	332-336 bp	1.0	82.9
psbC-trnS-UGA_p5	CATTTGTGGCATGCAGGAAG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.0	58.1	324-328 bp	1.0	82.9