Hotspot

trnE-UUC-trnT-GGU

LSC · 33226-34065 · 840 bp

Back to Kaempferia

Pi 0.0117
MarkerSeek score 65.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
840
variable_sites
18
pi
0.0117
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TCCTTTCGTAGTACCCCCAG GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 979-1065 bp 1.0 81.6
trnE-UUC-trnT-GGU_p2 TCCTTTCGTAGTACCCCCAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.1 58.1 958-1044 bp 1.0 81.2
trnE-UUC-trnT-GGU_p3 TCCTTTCGTAGTACCCCCAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.1 58.1 955-1041 bp 1.0 81.0
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 943-1029 bp 1.0 80.7
trnE-UUC-trnT-GGU_p5 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 922-1008 bp 1.0 80.6