Hotspot

petN-psbM

LSC · 30055-31334 · 1280 bp

Back to Kaempferia

Pi 0.0145

MarkerSeek score 59.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1280
variable_sites: 32
pi: 0.0145
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TATCCAGACTCACGAGGGTC	AGGGAAGACGATAGGGTTGG	55.0	55.0	57.9	58.5	2117-2185 bp	1.0	81.1
petN-psbM_p2	AGACTCACGAGGGTCTTTGA	AGGGAAGACGATAGGGTTGG	50.0	55.0	58.0	58.5	2112-2180 bp	0.75	70.2
petN-psbM_p3	CAGACTCACGAGGGTCTTTG	AGGGAAGACGATAGGGTTGG	55.0	55.0	57.9	58.5	2113-2181 bp	0.75	69.7
petN-psbM_p4	AGACTCACGAGGGTCTTTGA	CCAGTTCCAGTCCCAATTGA	50.0	50.0	58.0	57.4	2351-2419 bp	0.75	69.5
petN-psbM_p5	AGACTCACGAGGGTCTTTGA	TCCAGTTCCAGTCCCAATTG	50.0	50.0	58.0	57.4	2352-2420 bp	0.75	69.5