Hotspot

atpH-atpI

LSC · 14323-15526 · 1204 bp

Back to Kaempferia

Pi 0.0143

MarkerSeek score 58.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1204
variable_sites: 31
pi: 0.0143
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CTTCCGGTTGTCTCGCAATA	TCACGTCCGTCGCATATTTT	50.0	45.0	58.0	58.0	1654-2075 bp	1.0	85.4
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TCACGTCCGTCGCATATTTT	45.0	45.0	58.0	58.0	1553-1974 bp	1.0	85.2
atpH-atpI_p3	TACCTTCTACAGCTTGGCCT	TCACGTCCGTCGCATATTTT	50.0	45.0	58.0	58.0	1636-2057 bp	1.0	85.0
atpH-atpI_p4	CTTCCGGTTGTCTCGCAATA	ACATTCAACCAACCCCCATC	50.0	50.0	58.0	58.1	1591-2012 bp	1.0	84.9
atpH-atpI_p5	AAGTGCTAATGCCACAACCA	TCACGTCCGTCGCATATTTT	45.0	45.0	58.0	58.0	1743-2164 bp	0.75	74.7