Hotspot

trnS-GCU-trnR-UCU

LSC · 8800-10439 · 1640 bp

Back to Kaempferia

Pi 0.0158

MarkerSeek score 64.90

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1640
variable_sites: 47
pi: 0.0158
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1693-1795 bp	1.0	68.3
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1692-1794 bp	1.0	68.3
trnS-GCU-trnR-UCU_p3	AGTATCAAGCGGGTCGAGTA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1777-1879 bp	1.0	67.7
trnS-GCU-trnR-UCU_p4	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1699-1801 bp	1.0	67.6
trnS-GCU-trnR-UCU_p5	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1698-1800 bp	1.0	67.6