Hotspot

trnQ-UUG-psbK

LSC · 7455-7797 · 343 bp

Back to Kaempferia

Pi 0.0136

MarkerSeek score 57.50

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 343
variable_sites: 7
pi: 0.0136
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GAAGGATTCGAACCTCCGAG	ACTGTTCGAATAAAGGACAGA	55.0	38.1	57.8	54.6	458-467 bp	1.0	48.3
trnQ-UUG-psbK_p2	GAAGGATTCGAACCTCCGAG	AACTGTTCGAATAAAGGACAGA	55.0	36.4	57.8	55.4	459-468 bp	1.0	46.7
trnQ-UUG-psbK_p3	GAAGGATTCGAACCTCCGAG	ACTGTTCGAATAAAGGACAGAA	55.0	36.4	57.8	55.4	458-467 bp	1.0	46.7
trnQ-UUG-psbK_p4	AGGATTCGAACCTCCGAGTA	ACTGTTCGAATAAAGGACAGA	50.0	38.1	57.6	54.6	456-465 bp	1.0	46.5
trnQ-UUG-psbK_p5	AAGGATTCGAACCTCCGAGT	ACTGTTCGAATAAAGGACAGA	50.0	38.1	58.4	54.6	457-466 bp	1.0	46.4