Hotspot

ycf1

Genome · 125224-130725 · 5502 bp

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Pi 0.0151

MarkerSeek score 54.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5502
variable_sites: 187
pi: 0.0151
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCTTTTTCGGGGAGAACCAA	CTTGTTCTGGATGTGCTCGA	50.0	50.0	58.0	57.9	1263-1325 bp	1.0	83.5
ycf1_p2	CCTTTTTCGGGGAGAACCAA	TCTTGTTCTGGATGTGCTCG	50.0	50.0	58.0	57.9	1264-1326 bp	1.0	83.5
ycf1_p3	TTTCGGGGAGAACCAAAAGG	CTTGTTCTGGATGTGCTCGA	50.0	50.0	58.0	57.9	1259-1321 bp	1.0	83.5
ycf1_p4	TTTCGGGGAGAACCAAAAGG	TCTTGTTCTGGATGTGCTCG	50.0	50.0	58.0	57.9	1260-1322 bp	1.0	83.5
ycf1_p5	CAGCTTCCATTCCCCAGATC	CTTGTTCTGGATGTGCTCGA	55.0	50.0	58.0	57.9	1234-1296 bp	1.0	83.5