Hotspot

ndhD

Genome · 116843-118363 · 1521 bp

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Pi 0.00767
MarkerSeek score 49.40
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
Genome
length_bp
1521
variable_sites
25
pi
0.00767
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 GCTAATGAGGCATGGGGATC GCTATTGGGCCAGCTATTGT 55.0 50.0 58.2 58.0 2561-2766 bp 1.0 84.9
ndhD_p2 GGGAGCAGTATGGGCTAATG GCTATTGGGCCAGCTATTGT 55.0 50.0 57.8 58.0 2574-2779 bp 1.0 84.7
ndhD_p3 GCTAATGAGGCATGGGGATC CTGCTCCAAGAACAGAGGAC 55.0 55.0 58.2 57.9 2087-2294 bp 1.0 84.6
ndhD_p4 GTGTCATTGGCCTAGGGTTT GCTATTGGGCCAGCTATTGT 50.0 50.0 57.8 58.0 2620-2825 bp 1.0 84.5
ndhD_p5 GGGAGCAGTATGGGCTAATG CTGCTCCAAGAACAGAGGAC 55.0 55.0 57.8 57.9 2100-2307 bp 1.0 84.4