Hotspot

ndhF-rpl32

Genome · 113523-114360 · 838 bp

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Pi 0.0233

MarkerSeek score 68.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 838
variable_sites: 41
pi: 0.0233
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGCAGGAGTCCCAATCCTA	GTACGTTTCTTTGGAACCGC	50.0	50.0	57.7	57.7	583-1084 bp	1.0	82.8
ndhF-rpl32_p2	AGCAGGAGTCCCAATCCTAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	57.5	57.7	582-1083 bp	1.0	81.0
ndhF-rpl32_p3	AAGCAGGAGTCCCAATCCTA	TACGTTTCTTTGGAACCGCC	50.0	50.0	57.7	58.8	582-1083 bp	1.0	79.1
ndhF-rpl32_p4	AGCAGGAGTCCCAATCCTAT	TACGTTTCTTTGGAACCGCC	50.0	50.0	57.5	58.8	581-1082 bp	1.0	77.2
ndhF-rpl32_p5	GCAGGAGTCCCAATCCTATTG	GTACGTTTCTTTGGAACCGC	52.4	50.0	58.1	57.7	581-1082 bp	1.0	75.9