Hotspot

psbE-petL

Genome · 66010-67321 · 1312 bp

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Pi 0.0337

MarkerSeek score 60.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 1312
variable_sites: 67
pi: 0.0337
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.54
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.8	58.5	613-1382 bp	1.0	84.1
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.8	58.5	611-1380 bp	1.0	84.1
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	AAGCAGCCAGTAGAAAACCG	55.0	50.0	58.2	58.5	608-1377 bp	1.0	83.8
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GCAGCCAGTAGAAAACCGAA	55.0	50.0	58.2	58.5	606-1375 bp	1.0	83.8
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.4	58.5	606-1375 bp	1.0	82.6