Hotspot

ycf4-cemA

Genome · 61783-62781 · 999 bp

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Pi 0.00594

MarkerSeek score 67.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: Genome
length_bp: 999
variable_sites: 14
pi: 0.00594
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	GGGAAGGGAGTCAATGCTTT	45.0	50.0	57.9	57.8	932-1057 bp	1.0	82.7
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	GGGAAGGGAGTCAATGCTTT	50.0	50.0	58.1	57.8	934-1059 bp	1.0	82.4
ycf4-cemA_p3	GGAGACATTCCCTTGACTCG	GGGAAGGGAGTCAATGCTTT	55.0	50.0	57.7	57.8	1014-1139 bp	1.0	80.8
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	GGGAAGGGAGTCAATGCTTT	45.0	50.0	57.7	57.8	937-1062 bp	1.0	80.7
ycf4-cemA_p5	TGCGCGTACCAATTGAAGTA	CCCCCAGGGCAGAAATACTA	45.0	55.0	57.9	58.5	966-1091 bp	1.0	80.6