Hotspot

trnM-CAU-atpE

Genome · 53742-53961 · 220 bp

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Pi 0.0349

MarkerSeek score 74.50

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: Genome
length_bp: 220
variable_sites: 16
pi: 0.0349
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	314-322 bp	1.0	84.0
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	GCGAATCTAGCTCTCAGACG	50.0	55.0	58.4	58.0	324-332 bp	1.0	83.8
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	TGAGGCGAATCTAGCTCTCA	50.0	50.0	58.4	57.9	328-336 bp	1.0	83.5
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	AGACAAGCAATTGAGGCGAA	50.0	45.0	58.4	58.1	339-347 bp	1.0	82.9
trnM-CAU-atpE_p5	TATTGCTTTCATACGGCGGG	CTGAAGGCAAGAGACAAGCA	50.0	50.0	58.4	58.1	350-358 bp	1.0	82.6