Hotspot

petN-psbM

Genome · 29447-30487 · 1041 bp

Back to Kadsura

Pi 0.082

MarkerSeek score 87.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: Genome
length_bp: 1041
variable_sites: 199
pi: 0.082
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 87.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CTTGGGCGGCTTTAATGGTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	797-1191 bp	1.0	78.8
petN-psbM_p2	TTGGGCGGCTTTAATGGTAG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	796-1190 bp	1.0	78.8
petN-psbM_p3	GGCGGCTTTAATGGTAGTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.7	57.6	793-1187 bp	1.0	78.1
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	748-1142 bp	1.0	77.9
petN-psbM_p5	AGTAAATCTTGCTTGGGCGG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.5	57.6	808-1202 bp	1.0	76.5