Hotspot

atpH-atpI

Genome · 14308-15202 · 895 bp

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Pi 0.0265

MarkerSeek score 65.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 895
variable_sites: 52
pi: 0.0265
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAAGGC	45.0	50.0	58.0	57.0	713-956 bp	1.0	80.2
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TAGGTGAATCCATGGAAGGC	45.0	50.0	58.2	57.0	714-957 bp	1.0	79.0
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	GGTGAATCCATGGAAGGCC	45.0	57.9	58.0	58.2	711-954 bp	1.0	79.0
atpH-atpI_p4	TCCAGCAGCAATAACGGAAG	TAGGTGAATCCATGGAAGGC	50.0	50.0	58.3	57.0	723-966 bp	1.0	78.4
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	GGTGAATCCATGGAAGGCC	45.0	57.9	58.2	58.2	712-955 bp	1.0	77.8