Hotspot

rps16-trnQ-UUG

Genome · 6131-7800 · 1670 bp

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Pi 0.0211

MarkerSeek score 67.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 1670
variable_sites: 68
pi: 0.0211
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCATGTCGTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	800-1772 bp	1.0	86.3
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	788-1760 bp	1.0	85.8
rps16-trnQ-UUG_p3	ATCATGTCGTTCAAGTCGCA	AAAATCTAACTGGGGCGTGG	45.0	50.0	57.9	58.2	858-1830 bp	1.0	85.4
rps16-trnQ-UUG_p4	ATCATGTCGTTCAAGTCGCA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	57.9	57.8	804-1776 bp	1.0	85.2
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	AAAATCTAACTGGGGCGTGG	45.0	50.0	58.1	58.2	846-1818 bp	1.0	85.0