Hotspot

rpl16

LSC · 79630-81108 · 1479 bp

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Pi 0.051

MarkerSeek score 83.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1479
variable_sites: 195
pi: 0.051
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CAAATGGGTCTGGGGTTGAA	AGAATATCTTCCGGCTCCGA	50.0	50.0	58.0	58.0	2180-2457 bp	1.0	91.9
rpl16_p2	TTCAAATGGGTCTGGGGTTG	AGAATATCTTCCGGCTCCGA	50.0	50.0	58.0	58.0	2182-2459 bp	1.0	91.9
rpl16_p3	ACACATCAATTCTCGAGCCC	AGAATATCTTCCGGCTCCGA	50.0	50.0	58.0	58.0	2219-2496 bp	0.909091	88.1
rpl16_p4	CAAATGGGTCTGGGGTTGAA	CAAGTACAAATTGCAGGGCG	50.0	50.0	58.0	58.0	1944-2130 bp	0.636364	75.4
rpl16_p5	TTCAAATGGGTCTGGGGTTG	CAAGTACAAATTGCAGGGCG	50.0	50.0	58.0	58.0	1946-2132 bp	0.636364	75.4