Hotspot

psbE-petL

LSC · 63625-64531 · 907 bp

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Pi 0.0375

MarkerSeek score 82.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 103
pi: 0.0375
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	ACCGAAATAACTAGTTATAGTAAGCA	50.0	30.8	57.8	56.1	892-961 bp	1.0	52.0
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	ACCGAAATAACTAGTTATAGTAAGCA	55.0	30.8	58.2	56.1	887-956 bp	1.0	52.0
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	ACCGAAATAACTAGTTATAGTAAGCA	50.0	30.8	58.4	56.1	885-954 bp	1.0	52.0
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AACCGAAATAACTAGTTATAGTAAGCA	50.0	29.6	57.8	56.7	893-962 bp	1.0	52.0
psbE-petL_p5	TTCTCCTGTGCTTCCAGACA	ACCGAAATAACTAGTTATAGTAAGCA	50.0	30.8	58.6	56.1	886-955 bp	1.0	52.0