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petA-psbJ

LSC · 61897-62835 · 939 bp

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Pi 0.0522

MarkerSeek score 82.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 939
variable_sites: 135
pi: 0.0522
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	CACGGACTAGCTGTACCAAC	50.0	55.0	58.0	58.0	1500-1609 bp	1.0	91.4
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1404-1506 bp	1.0	91.3
petA-psbJ_p3	TCGAGGAAGGGGTCAGATTT	CACGGACTAGCTGTACCAAC	50.0	55.0	58.0	58.0	1781-1890 bp	1.0	90.6
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	CCTATTTATTGCGGGCTGGT	50.0	50.0	58.0	58.0	1738-1840 bp	1.0	90.6
petA-psbJ_p5	TCGAGGAAGGGGTCAGATTT	CCTATTTATTGCGGGCTGGT	50.0	50.0	58.0	58.0	2019-2121 bp	1.0	90.0