Hotspot

accD-psaI

LSC · 57610-58236 · 627 bp

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Pi 0.0475

MarkerSeek score 81.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 627
variable_sites: 78
pi: 0.0475
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACCCGTTTGATCGAGTACG	TTGCAGCCAACAAGAATCCT	50.0	45.0	58.0	58.0	1549-1806 bp	1.0	91.6
accD-psaI_p2	AACCCGTTTGATCGAGTACG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	992-1234 bp	1.0	91.3
accD-psaI_p3	TGGTTCCTCTGGATCCCATT	TTGCAGCCAACAAGAATCCT	50.0	45.0	58.0	58.0	1748-2005 bp	1.0	91.1
accD-psaI_p4	ATTTTATTCGGCGGAGGACC	TTGCAGCCAACAAGAATCCT	50.0	45.0	58.0	58.0	1726-1983 bp	1.0	91.0
accD-psaI_p5	TGGATTTTATTCGGCGGAGG	TTGCAGCCAACAAGAATCCT	50.0	45.0	58.0	58.0	1729-1986 bp	1.0	90.9