Hotspot

trnS (UGA)-psbZ

LSC · 34969-35286 · 318 bp

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Pi 0.0444

MarkerSeek score 70.80

Primer available yes

Label trnS (UGA)-psbZ

Feature Metadata

label_name: trnS (UGA)-psbZ
feature_type: igs
region: LSC
length_bp: 318
variable_sites: 39
pi: 0.0444
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS (UGA)-psbZ_p1	CAACCACTCAGCCATCTCTC	AATCCTGTCTCCGCAACATC	55.0	50.0	58.0	58.0	986-1059 bp	1.0	91.6
trnS (UGA)-psbZ_p2	CAACCACTCAGCCATCTCTC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.0	58.1	828-882 bp	1.0	91.4
trnS (UGA)-psbZ_p3	CAACCACTCAGCCATCTCTC	CCGGAGAAGCAAATACGACA	55.0	50.0	58.0	58.0	416-430 bp	1.0	90.4
trnS (UGA)-psbZ_p4	CAACCACTCAGCCATCTCTC	GTCGGGCTAGAAGACTCTCT	55.0	55.0	58.0	58.0	727-751 bp	0.818182	84.2
trnS (UGA)-psbZ_p5	CAACCACTCAGCCATCTCTC	GCTCTGTTAACTAGTGGGGC	55.0	55.0	58.0	58.0	1007 bp	0.090909	43.3