Hotspot

trnE (UUC)-trnT (GGU)

LSC · 30207-30835 · 629 bp

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Pi 0.0584

MarkerSeek score 87.90

Primer available yes

Label trnE (UUC)-trnT (GGU)

Feature Metadata

label_name: trnE (UUC)-trnT (GGU)
feature_type: igs
region: LSC
length_bp: 629
variable_sites: 92
pi: 0.0584
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 87.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE (UUC)-trnT (GGU)_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	690-934 bp	1.0	90.7
trnE (UUC)-trnT (GGU)_p2	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	653-897 bp	1.0	90.6
trnE (UUC)-trnT (GGU)_p3	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	687-931 bp	1.0	89.5
trnE (UUC)-trnT (GGU)_p4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	650-894 bp	1.0	89.4
trnE (UUC)-trnT (GGU)_p5	TTTCGTAGTACCCTACCCCC	TTGAACCGATGACTTACGCC	55.0	50.0	57.8	58.3	692-936 bp	1.0	88.5