Hotspot

rpoB-trnC (GCA)

LSC · 26268-27375 · 1108 bp

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Pi 0.053

MarkerSeek score 89.40

Primer available yes

Label rpoB-trnC (GCA)

Feature Metadata

label_name: rpoB-trnC (GCA)
feature_type: igs
region: LSC
length_bp: 1108
variable_sites: 163
pi: 0.053
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 89.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC (GCA)_p1	AGTTCTTCTGTCAAACCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1274-1456 bp	1.0	89.3
rpoB-trnC (GCA)_p2	GTCAAACCCCGGTCAATGAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1265-1447 bp	1.0	88.2
rpoB-trnC (GCA)_p3	TCAAACCCCGGTCAATGAAC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1264-1446 bp	1.0	88.2
rpoB-trnC (GCA)_p4	CCCGGTCAATGAACCTACAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1258-1440 bp	1.0	87.5
rpoB-trnC (GCA)_p5	AGTTCTTCTGTCAAACCCCG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1256-1438 bp	1.0	87.1