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rps16-trnQ (UUG)

LSC · 5666-6544 · 879 bp

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Pi 0.062

MarkerSeek score 82.30

Primer available yes

Label rps16-trnQ (UUG)

Feature Metadata

label_name: rps16-trnQ (UUG)
feature_type: igs
region: LSC
length_bp: 879
variable_sites: 145
pi: 0.062
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ (UUG)_p1	GAGCACCCTCATCCCTAGAT	AGCAGCTTGCCAAACAAAAG	55.0	45.0	58.0	58.1	1266-1593 bp	0.909091	87.7
rps16-trnQ (UUG)_p2	GAGCACCCTCATCCCTAGAT	CTTACAGCAGCTTGCCAAAC	55.0	50.0	58.0	57.9	1271-1598 bp	0.909091	87.7
rps16-trnQ (UUG)_p3	GAGCACCCTCATCCCTAGAT	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.0	58.1	691-1019 bp	0.909091	87.6
rps16-trnQ (UUG)_p4	GAGCACCCTCATCCCTAGAT	AAAAGCACAGGTACCACTGG	55.0	50.0	58.0	58.0	1562 bp	0.090909	43.3
rps16-trnQ (UUG)_p5	ATTGAGCAATTCCAACCGGT	AAAAGCACAGGTACCACTGG	45.0	50.0	58.1	58.0	1794 bp	0.090909	42.9