Hotspot

trnK-UUU-rps16

LSC · 4071-4532 · 462 bp

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Pi 0.0627
MarkerSeek score 81.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
462
variable_sites
93
pi
0.0627
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GCCGTACGAGGAGAAAACTT 50.0 50.0 58.2 57.9 739-772 bp 0.909091 86.4
trnK-UUU-rps16_p2 GGGAGAAGGTTTTTGGGGTT AGCCGTCTATCGAATCGTTG 50.0 50.0 57.9 57.9 1386-1422 bp 0.545455 68.9
trnK-UUU-rps16_p3 GGGAGAAGGTTTTTGGGGTT TCGATCCCGAAGAGAAGGAA 50.0 50.0 57.9 57.9 1356-1392 bp 0.545455 68.9
trnK-UUU-rps16_p4 GGGAGAAGGTTTTTGGGGTT GCCGTACGAGGAGAAAACTT 50.0 50.0 57.9 57.9 1459-1490 bp 0.454545 65.1
trnK-UUU-rps16_p5 GGGAGAAGGTTTTTGGGGTT TCTATCCCAATGAGCCGTCT 50.0 50.0 57.9 57.9 1398-1434 bp 0.363636 60.7