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matK-trnK-UUU

LSC · 3306-4033 · 728 bp

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Pi 0.0542

MarkerSeek score 83.70

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 104
pi: 0.0542
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACCAAGATTTCCACGTGGAC	AGCCGTCTATCGAATCGTTG	50.0	50.0	58.1	57.9	1854-1923 bp	1.0	90.2
matK-trnK-UUU_p2	CCAAGATTTCCACGTGGACA	AGCCGTCTATCGAATCGTTG	50.0	50.0	58.1	57.9	1853-1922 bp	1.0	90.2
matK-trnK-UUU_p3	ACCAAGATTTCCACGTGGAC	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.1	57.9	1824-1893 bp	1.0	90.0
matK-trnK-UUU_p4	ACCAAGATTTCCACGTGGAC	TCTATCCCAATGAGCCGTCT	50.0	50.0	58.1	57.9	1866-1935 bp	0.818182	83.2
matK-trnK-UUU_p5	CCAAGATTTCCACGTGGACA	TCTATCCCAATGAGCCGTCT	50.0	50.0	58.1	57.9	1865-1934 bp	0.818182	83.1