Hotspot

ycf1

SSC · 108089-113302 · 5214 bp

Back to Jurinea

Pi 0.00754

MarkerSeek score 49.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5214
variable_sites: 75
pi: 0.00754
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTTGAAACAAAGACTAGATTGTC	TCTGTGCAATTTTCTATCGAAGT	33.3	34.8	55.3	56.5	207-213 bp	1.0	44.5
ycf1_p2	ACTTGAAACAAAGACTAGATTGTC	TCGAGAATTAGGATAGGGAACA	33.3	40.9	55.3	55.8	262-268 bp	1.0	43.6
ycf1_p3	ACTTGAAACAAAGACTAGATTGT	TCGAGAATTAGGATAGGGAACA	30.4	40.9	54.2	55.8	262-268 bp	1.0	43.6
ycf1_p4	ACTTGAAACAAAGACTAGATTGTC	TCGAGAATTAGGATAGGGAACAAG	33.3	41.7	55.3	57.6	262-268 bp	1.0	43.6
ycf1_p5	ACTTGAAACAAAGACTAGATTGTC	CGAGAATTAGGATAGGGAACAAGG	33.3	45.8	55.3	58.5	261-267 bp	1.0	43.6