Hotspot

ccsA

SSC · 121770-122738 · 969 bp

Back to Jurinea

Pi 0.00516

MarkerSeek score 62.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 10
pi: 0.00516
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGAACCGCGTGAATCACTAC	GCGGCATGCCATCTTATAAA	50.0	45.0	58.0	56.6	1198-1232 bp	1.0	69.6
ccsA_p2	TGATTCACATGGTTCTCGCC	GCGGCATGCCATCTTATAAA	50.0	45.0	58.3	56.6	1172-1206 bp	1.0	67.6
ccsA_p3	GAGAACCGCGTGAATCACTA	GCGGCATGCCATCTTATAAA	50.0	45.0	57.7	56.6	1199-1233 bp	1.0	67.5
ccsA_p4	AGAACCGCGTGAATCACTAC	GCGGCATGCCATCTTATAAAA	50.0	42.9	58.0	57.4	1198-1232 bp	1.0	67.5
ccsA_p5	GAACCGCGTGAATCACTACA	GCGGCATGCCATCTTATAAA	50.0	45.0	58.3	56.6	1197-1231 bp	1.0	67.3