Hotspot

psaI-ycf4

LSC · 58732-59138 · 407 bp

Back to Jurinea

Pi 0.00251

MarkerSeek score 58.70

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 407
variable_sites: 2
pi: 0.00251
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCAATCGCAATGGCTTCTTT	TACTTGTTTTTCGAGCCCCC	45.0	50.0	58.0	58.1	512-522 bp	1.0	81.1
psaI-ycf4_p2	GCAATCGCAATGGCTTCTTT	GGATAACAGCCCAGCAGAAA	45.0	50.0	58.0	57.9	533-543 bp	1.0	80.8
psaI-ycf4_p3	GCAATCGCAATGGCTTCTTT	AAAGGATAACAGCCCAGCAG	45.0	50.0	58.0	57.9	536-546 bp	1.0	80.7
psaI-ycf4_p4	AGTAGGCCTAGTCTTTCCGG	TACTTGTTTTTCGAGCCCCC	55.0	50.0	58.2	58.1	531-541 bp	1.0	79.2
psaI-ycf4_p5	AGTAGGCCTAGTCTTTCCGG	GGATAACAGCCCAGCAGAAA	55.0	50.0	58.2	57.9	552-562 bp	1.0	78.9