Hotspot

rpoB-rpoC1

LSC · 15620-15645 · 26 bp

Back to Jurinea

Pi 0.0385

MarkerSeek score 62.30

Primer available yes

Label rpoB-rpoC1

Feature Metadata

label_name: rpoB-rpoC1
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 2
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rpoC1_p1	TTGCTCGTTCGAGAACTACG	CACCTCTCCAACTATCTCGC	50.0	55.0	58.0	57.5	230 bp	1.0	76.4
rpoB-rpoC1_p2	TTGCTCGTTCGAGAACTACG	ACCTCTCCAACTATCTCGCC	50.0	55.0	58.0	58.6	229 bp	1.0	75.6
rpoB-rpoC1_p3	TTGCTCGTTCGAGAACTACG	CCTCTCCAACTATCTCGCCA	50.0	55.0	58.0	58.6	228 bp	1.0	75.6
rpoB-rpoC1_p4	GGTTGCTCGTTCGAGAACTA	CACCTCTCCAACTATCTCGC	50.0	55.0	57.7	57.5	232 bp	1.0	73.9
rpoB-rpoC1_p5	GGTTGCTCGTTCGAGAACTA	ACCTCTCCAACTATCTCGCC	50.0	55.0	57.7	58.6	231 bp	1.0	73.2